Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. International Journal of Biological Macromolecules, 23, 135-141. sharing sensitive information, make sure youre on a federal Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Cookies policy. Journal of Applied Polymer Science, 27, 795-810. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Hydrodynamic Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. ; Hogan, M.P. The intrinsic viscosity calculation requires several concentrations, and because of this inconvenient will not use the Huggins method in this paper. (2012). Biotechnology Progress, 19, 544-548. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. He, L.; Niemeyer, B. ; Wood, P.J. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Harding, Stephen E. (2005). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. (2003). Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. NCI CPTC Antibody Characterization Program. eCollection 2018 Feb. Molecules. Guner, Ali (1999). TEWL measurements were taken 30min after microporation of the human and pig skin. 2004;25(9):155967. Increased TEWL values are evidence of micropores formation. The mechanism of the dextran-induced red blood cell Aggregation. ; Yevlampieva N.P. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. 151-166. All branched polysaccharide types could be described approximately by the hyperbranching theory. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. 2010;4(1):117. WebTry out PMC Labs and tell us what you think. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Elias, Hans-Georg (1975). 7 8 Smith, David R.; Raymonda, John W. (1972). (2001). J. Braz. Gekko, K. (1971). J Cosmet Laser Ther. The Viscosity of Dilute Solutions of Long-Chain Molecules. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. The term f/f0 is sometimes denoted as P, Perrin constant. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Progr. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Fluorescent and Biotinylated DextransSection 14.5 Epub 2012 May 28. Regulating dynamic signaling between hematopoietic stem cells 8. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Increased epidermal laser fluence through simultaneous ultrasonic microporation. The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Anal. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. @ Nilimesh See attached. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Carbohydrate Polymers 95 360-365. After 24h, the cumulative permeation of FITC-dextran M.W. This situation is mainly the result of using dextans, solvent, additives, etc. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). (2008). Pathan IB, Setty CM. The human skins were from the back or thigh of a 24-year-old woman. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. 2018;1:10915. Durand, A. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Determination of intrinsic viscosities of macromolecules and nanoparticles. ; Prasad, D.H.L. Bohdanecky, Miloslav (1996). Expert Rev Med Devices. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. (1954). Curvale, R.; Masuelli, M.; Perez Padilla, A. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. A solvent at this temperature is called a theta solvent [53]. ; Garca de , J. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. Naik A, Kalia YN, Guy RH. Carbohydrate Polymers, 78, 902-907. Drug Deliv. 2008;28(5):64557. Regulating dynamic signaling between hematopoietic stem cells Hrabchak C, Flynn L, Woodhouse KA. dextran Recent Pat. (1969). II. Alternatives to animal testing: a review. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Epub 2017 Jun 1. Dilute Solution. (2003). Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. 43-1, 115-147. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Die Makromolekulare Chemie, 148(1), 229-238. This conjecture found to be correct. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Arch Dermatol Res. If there are particles in the sample, the incident laser light gets scattered in all directions. (2013). The hydrodynamic 57 radius of the dextranase is 7 The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Progress in Biophysical Molecules Biological, 68, 207-262. 2009;8(2):1739. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Provided by the Springer Nature SharedIt content-sharing initiative. Epub 2022 Feb 2. Unable to load your collection due to an error, Unable to load your delegates due to an error. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. The micropores of the human cadaver skin were regenerated according to the time. Walkenhorst, S.; Olivier, R. (2003). dextran Carbohydr Polym. 2013;43(4):287303. J. Braz. Hydrodynamic At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. Webof overall macromolecular geometry and hydrodynamic draining. Study of temperature influence. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). H&E stained images were analyzed using Image J software. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". ; Bataille, B. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Theory, Estimation, Experiment, and Data. An undergraduate physical chemistry experiment. (1991). Masuelli, Martin Alberto. Huggins equation [41] gives the relation to the relative viscosity increase (i). The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. It is well tolerated on parenteral infusion by most people but a very low incidence What is hydrodynamic radius WebDextran produced from Weissella cibaria JAG8 was purified and characterized. 2. Cell permeabilization using a non-thermal plasma - IOPscience Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Polymers | Free Full-Text | Confinement-Induced Fractionation and These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. (1958). Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. Indo AM J Pharm. Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Transdermal drug delivery: overcoming the skins barrier function. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). 4, 10, and 20K) was measured using the Transdermal diffusion cell system. 4h after microporation, the sizes of the micropore decreased slightly. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. Dextrans in Aqueous Solution. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. The Mark-Houwink a constant is close to 0.5 in poor solvents. Progress in Polymer Science, 30, 38-70. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Pharm Res. Banga AK. 2008;26(11):12618. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). WebDextran 40 Pharmaceutical Quality. Polyelectrolytes as secondary calibration standards for aqueous SEC. 2015;23(3):2239. Ther. The solution was measured using an Anton Paar DMA35N densitometer. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Carbohydrate Polymers, 36, 319-326. Structural and biocompatibility properties of dextran from In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. Polysaccharide hydrogels for modified release formulations. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. The .gov means its official. Viswanath, D.S. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Nordmeier, Eckliard (1993). Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Hydrodynamic Properties of Tragacanthin. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size.

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